L-VIS (IN-VITRO SLICE CHAMBER)

$499.00

PopNeuron’s Ultra-Low Volume In-Vitro Slice Chamber marks a significant advancement in neuroscience research. This innovative chamber requires only 1.5 to 2 ml of artificial cerebro-spinal fluid (ACSF) for experiments, drastically reducing the volume compared to other designs. By eliminating external containers and tubes, it facilitates continuous reoxygenation and recirculation of ACSF directly in the chamber, ensuring efficient oxygen supply and cost savings, especially when using expensive pharmacological additives. Rigorously tested through fluid dynamics modeling, real-life experiments with dyes and beads, and comprehensive tissue health assessments, this chamber offers a practical, cost-effective solution for intricate neuroscience studies, maximizing the potential of live tissue analysis.

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L-VIS (Ultra-Low Volume In-Vitro Slice Chamber)

Many experimental approaches in medicine or biology, and particularly in neuroscience, require the use of live tissue. Ex-vivo tissue samples, such as brain slices, are prepared and then kept alive in artificial cerebro-spinal fluid (ACSF), a solution containing the most important ingredients of actual cerebro-spinal fluid. Additionally, it has sugar added as an energy source and is bubbled with oxygen to keep the tissue alive. While the tissue is under a microscope for examination, for example, through patch clamp recordings, it is continuously bathed in ACSF, which is rapidly exchanged to ensure there is always enough oxygen available for the tissue. From a practical point of view, the chamber in which the tissue is located (the recording chamber) has an inflow and an outflow connected with tubes to holding containers for continuous fluid exchange.

In its simplest form, ACSF contains just a few inexpensive ingredients, and even liters of it cost very little to make. Therefore, discarding the solution after it has been used in the recording chamber is not a problem. However, whenever pharmacological ingredients such as ion channel blockers, receptor blockers, or similar reagents are added to the solution, expenses can increase significantly – to the point where just one day of recording can cost hundreds or even thousands of dollars. In these cases, investigators try to reduce the amount of ACSF as much as possible – for example, by designing smaller containers or reducing flow rates. Additionally, they recycle the solution rather than discarding it after a single pass through the recording chamber.

PopNeuron’s L-VIS takes this concept to the limit of what is possible. Our chamber uses about 1.5 to 2 ml of ACSF per experiment, which is several-fold less than any other design on the market or what can be custom-designed by investigators. The main reason for the small volume is that we eliminated external containers and tubes with the solution altogether, taking a literal approach to “out of the box” thinking. Our chamber requires a single fill with ACSF. The solution is then continuously reoxygenated through a small tube that carries just the gas into the chamber. A water pump design recirculates ACSF within the chamber, mixes and reoxygenates the solution, and moves it across the tissue in a figure-8 movement pattern.

How do we know this design works?

The fluid dynamics were modeled, and the chamber design was adjusted in multiple iterative steps. Once a design looked feasible, a prototype was produced and tested in real life with dyes and small beads transported in the fluid. Videos of these motions were carefully analyzed, and the results were used to further optimize the design, followed by more modeling and more real-life testing. Oxygen saturation of the solution was tested over multiple hours to measure the ability of the water pump mechanisms to oxygenate the solution. Liquid chromatography and mass spectroscopy were used to test for potential changes in the solution over time and control for possible accumulation of waste products. Finally, patch clamp recordings were performed over several hours to test for the overall health of the tissue.

 

 

Additional information

Color

Anodized Silver

Dimensions

10cm x 4 cm x 2cm

Weight

.2 kg

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